Intermediate

Part:BBa_K1230014:Design

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-26)


rbs-GFP-pLacI-rbs-MarA Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1266
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 665


Design Notes

Intermediate part.


Source

2013 DNA distribution. Genscript

References

1. Maneewannakul, K. & Levy, S. B. (1996). Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli. Antimicrob Agents Chemother 40, 1695–1698. 2. Warner, D.M.& Levy, S.B., "Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon", Microbiology, vol. 156, 2010, p.570-578. 3. Nikaido, H. (2003). Bile salts and fatty acids induce the expression of Escherichia coli AcrAB multidrug efflux pump through their interaction with Rob regulatory protein. Mol Microbiol 48, 1609–1619. 4. Wandersman, C. & Delepelaire, P. (1990). TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc Natl Acad Sci U S A 87, 4776–4780.